Integrate a broad suite of biophysical and functional assays to comprehensively profile the Fc function of immune effectors. Using multidimensional data and targeted analysis, we help teams assess effector function, understand mechanism of action, and guide development decisions.
Advantages of SeromYx Assays:
- Physiologically relevant systems
- Primary cell–based readouts
- Antigen-specific designs
- High-throughput capacity
- Scalable to thousands of samples in a single run
- Flexible across diverse antigens, formats, matrices, cell types, and species
BIOPHYSICAL ASSAYS
Fc Receptor Binding Array
Fluorescently coded microspheres capture multiple antigen specificities simultaneously and profile the effector capacity by assessing interaction of antigen-specific antibodies with Fc receptors. Available receptors: FCGR2A (R131, H131), FCGR2B, FCGR3A (V158, F158), FCGR3B, FCRN (pH 6.0, 7.4), FCA, C1q, TRIM21.
Antigen Specific Antibody Isotyping and Subclassing (ISSC)
Fluorescently coded microspheres capture multiple antigen specificities simultaneously and profile the isotype/subclass distribution in an antigen-specific manner. Available receptors: Total IgG, IgG1, IgG2, IgG3, IgG4, IgA1, IgA2, IgM.
Antibody Avidity by SPR
Avidity Index measures the strength of binding to a target antigen by either polyclonal antibodies from serum or monoclonal antibody cocktails. The off-rate is utilized along with the magnitude of
response to compute avidity index.
Antibody Affinity by SPR
Tripartite binding measures traditional antibody affinity kinetics in an antigen-specific manner. The kinetics of the antigen-antibody-receptor interaction describe the on-rate, the off-rate, and the associated affinity (equilibrium dissociation constant, KD) of the antibody with the receptor. We also conduct bipartite Fc-FcR affinity measurements.
CELLULAR FUNCTIONAL ASSAYS
Antibody Dependent Cellular Phagocytosis (ADCP)
Assesses the ability of antibodies to induce phagocytosis of antigen- functionalized fluorescent beads by monocytes via Fc receptors.
Antibody Dependent Neutrophil Phagocytosis (ADNP)
Assesses the ability of antibodies to induce the phagocytosis of antigen-coated targets by primary neutrophils.
Antibody Dependent Basophil Phagocytosis (ADBP)
Assesses the ability of antibodies to induce the phagocytosis of antigen-coated targets by primary basophils.
Antibody Dependent Eosinophil Phagocytosis (ADEP)
Assesses the ability of antibodies to induce the phagocytosis of antigen-coated targets by primary eosinophils.
Assesses the ability of antibodies to induce phagocytosis of antigen-coated targets by dendritic cells as well as DC activation/maturation and cytokine release.
Antibody Dependent NK Cell
Activation (ADNKA)
Assesses the ability of antibodies to induce NK cell activation against antigen-coated plates by measuring the levels of CD107a, IFN-γ and MIP-1β.
Antibody Dependent Cellular
Cytotoxicity (ADCC)
Tests the ability of antigen-specific antibodies to recruit NK cell lytic activity.
Antibody Dependent Complement Deposition (ADCD)
Assesses the recruitment of complement component C3b on the surface of antigen-coupled beads.
Antibody Dependent Mucin
Binding (ADMB)
Measures the capacity of antibodies to trap pathogens in mucus proteins.
Antibody Dependent Enhancement
of Infection (ADEI)
Measures the enhancement of viral infection caused by antigen specific antibodies in non-susceptible target cells.
TAILORED FOR ANTIBODY THERAPEUTICS AND VACCINES
Our technology is designed to support a broad range of discovery and development efforts for both antibodies and vaccines.
When More Isn’t Better: Why Antibody Titers Alone Don’t Predict Protection
- Higher neutralization titer doesn’t always mean better protection: A study on the live-attenuated influenza vaccine shows no correlation between increased antibody levels and improved immunity.
- Neutralization titer increase does not guarantee influenza protection: Despite a tenfold rise in titer, the rate of influenza positivity remained unchanged.
- Functional assays are essential: Measuring antibody levels alone isn’t enough; functional assays are needed to assess true immune protection.
CD20 mAbs: How Fc Design Shapes Complement-Mediated Function
- Comparative Functional Profiling of CD20 mAbs: Rituximab, Ofatumumab, and Obinutuzumab exhibit distinct profiles in C1q binding, ADCD, and CDC activities, enabling developers to evaluate complement-related mechanisms across therapeutic antibodies targeting the same antigen.
- Ofatumumab Shows Highest Complement Activation Potential: Among the three CD20 mAbs, Ofatumumab demonstrates the strongest C1q binding and ADCD response, suggesting enhanced CDC potency and potential for superior direct complement-mediated cytotoxicity.
- Obinutuzumab’s Fc Engineering Impacts Complement Function: Obinutuzumab, an afucosylated Type II mAb, shows significantly reduced C1q binding and CDC despite strong ADCP, emphasizing the importance of Fc design choices on effector function profiles.
