The Systems Serology Platform
The Systems Serology Platform
Systems Serology is a suite of bead- and plate-based assays linked to systems biology/machine learning algorithms that enable the collection and interpretation of population-level functional and biophysical antibody profiles.
Developed at the Ragon Institute, Systems Serology assays define the specific characteristics of the most protective humoral immune responses in both monoclonal antibodies and polyclonal pools of antibodies via Fc effector function assays and links function back to biophysical antibody features.
Currently support human, murine, and NHP samples; other species on request.
Easily adaptable to analyze antibody responses against any antigen, both known and unknown.
SeromYx is fully GCLP-accredited, and assays are conducted under GCLP defined standards, supported by reports detailing assay sensitivity, reproducibility and performance.
Systems Serology 5-step Process
STEP ONE: SAMPLE COLLECTION
Samples can be serum or plasma, or a panel of monoclonal antibodies. Serum or plasma can be from immune, recovered or treated subjects over time, as well as case controls.
STEP TWO: RUN ASSAYS
Assay selection from the full suite of 12 assays is customized for each project depending on the client’s need for insight into specific humoral function and antibody characteristics. SeromYx is blinded during assay runs.
STEP THREE: COLLECT AND PROVIDE DATA
Provide raw data from the tests (hundreds of data points/sample) to client.
STEP FOUR: ANALYZE DATA
SeromYx is unblinded and applies its computational biology approaches to interpreting the data, often working closely with client statistical staff and incorporating additional data such as titers provided by the client.
STEP FIVE: INTERPRET AND SHARE RESULTS
Examples of actionable insight:
- Discovery – What function correlates with efficacy?
- Development – What construct delivers that function?
System Serology Assay Suite
SeromYx’ Systems Serology platform contains a suite of 12 high throughput assays. Nine assays interrogate antibody function and three assays describe antibody biophysical structure and thus link the specified effector function(s) back to the biophysical structure of the Fc region of the antibody. For more information on effector function and how the Fc region of antibodies drives it, please click here.
antibody dependent cellular phagocytosis
antibody dependent cellular cytotoxicity
antibody dependent NK cell activation
antibody dependent eosinophil degranulation
antibody dependent basophil degranulation
antibody dependent complement deposition
antibody dependent neutrophil activation/phagocytosis
antibody dependent dendritic cell phagocytosis
antibody dependent mucin binding
Fc Receptor Array
antigen-specific antibody Fc receptor binding
Antibody Glycosylation Analysis
antigen-specific glycosylation analysis
Antibody Subclassing & Isotyping
antigen-specific subclassing & isotyping