The Systems Serology Platform 

The Systems Serology Platform 

Systems Serology is a suite of bead- and plate-based assays linked to systems biology/machine learning algorithms that enable the collection and interpretation of population-level functional and biophysical antibody profiles.

Developed at the Ragon Institute, Systems Serology assays define the specific characteristics of the most protective humoral immune responses in both monoclonal antibodies and polyclonal pools of antibodies via Fc effector function assays and links function back to biophysical antibody features.

MULTI-SPECIES

Currently support human, murine, and NHP samples; other species on request.

ADAPTABLE

 Easily adaptable to analyze antibody responses against any antigen, both known and unknown.

QUALITY 

SeromYx is fully GCLP-accredited, and assays are conducted under GCLP defined standards, supported by reports detailing assay sensitivity, reproducibility and performance.

Systems Serology 5-step Process

STEP ONE: SAMPLE COLLECTION

Samples can be serum or plasma, or a panel of monoclonal antibodies. Serum or plasma can be from immune, recovered or treated subjects over time, as well as case controls.

STEP TWO: RUN ASSAYS

Assay selection from the full suite of 12 assays is customized for each project depending on the client’s need for insight into specific humoral function and antibody characteristics. SeromYx is blinded during assay runs.

STEP THREE: COLLECT AND PROVIDE DATA

Provide raw data from the tests (hundreds of data points/sample) to client.

STEP FOUR: ANALYZE DATA

SeromYx is unblinded and applies its computational biology approaches to interpreting the data, often working closely with client statistical staff and incorporating additional data such as titers provided by the client.

STEP FIVE: INTERPRET AND SHARE RESULTS

Examples of actionable insight:

  • Discovery – What function correlates with efficacy? 
  • Development – What construct delivers that function? 

System Serology Assay Suite

SeromYx’ Systems Serology platform contains a suite of 12  high throughput assays. Nine assays interrogate antibody function and three assays describe antibody biophysical structure and thus link the specified effector function(s) back to the biophysical structure of the Fc region of the antibody. For more information on effector function and how the Fc region of antibodies drives it, please click here.

ADCP

antibody dependent cellular phagocytosis

ADCC

antibody dependent cellular cytotoxicity

ADNKA

antibody dependent NK cell activation

ADED

antibody dependent eosinophil degranulation

ADBD

antibody dependent basophil degranulation

ADCD

antibody dependent complement deposition

ADNP

antibody dependent neutrophil activation/phagocytosis

ADDCP

antibody dependent dendritic cell phagocytosis

ADMB

antibody dependent mucin binding

Fc Receptor Array

antigen-specific antibody Fc receptor binding

Antibody Glycosylation Analysis

antigen-specific glycosylation analysis

Antibody Subclassing & Isotyping

antigen-specific subclassing & isotyping

Let's Talk - Contact us today to discuss your project.